Includes index.

1. Recommendations for Working with Pathogenic Bacteria / Stephen A. Morse and Joseph E. McDade -- 2. Determination of Median Lethal and Infectious Doses in Animal Model Systems / Susan Welkos and Alison O'Brien -- 3. Serogroup and Serotype Classification of Bacterial Pathogens / Carl E. Frasch -- 4. Analysis of Genetic Variation by Polymerase Chain Reaction-Based Nucleotide Sequencing / Kimberlyn Nelson and Robert K. Selander -- 5. Determinations of Restriction Fragment Length Polymorphism in Bacteria Using Ribosomal RNA Genes / Rivka Rudner, Barbara Studamire and Erich D. Jarvis -- 6. DNA Fingerprinting of Myobacterium tuberculosis / Dick Van Soolingen, Petra E. W. de Haas and Peter W. M. Hermans [et al.] -- 7. Phylogenetic Identification of Uncultured Pathogens Using Ribosomal RNA Sequences / Thomas M. Schmidt and David A. Relman -- 8. Isolation of Outer Membranes / Hiroshi Nikaido -- 9. Isolation and Characterization of Lipopolysaccharides, Lipooligosaccharides, and Lipid A / Michael A. Apicella, J. McLeod Griffiss and Herman Schneider -- 10. Isolation of Peptidoglycan and Soluble Peptidoglycan Fragments / Raoul S. Rosenthal and Roman Dziarski -- 11. Purification of Streptococcal M Protein / Vincent A. Fischetti -- 12. Purification of Escherichia coli K Antigens / Willie F. Vann and Stephen J. Freese -- 13. Purification of Somatic Pili / Gary K. Schoolnik -- 14. Detection, Isolation, and Characterization of Siderophores / Shelley M. Payne -- 15. Identification of Receptor-Mediated Transferrin-Iron Uptake Mechanism in Neisseria gonorrhoeae / Cynthia Nau Cornelissen and P. Frederick Sparling -- 16. Analysis and Construction of Stable Phenotypes in Gram-Negative Bacteria with Tn5- and Tn10-Derived Minitransposons / Victor de Lorenzo and Kenneth N. Timmis -- 17. Use of Transposons to Dissect Pathogenic Strategies of Gram-Positive Bacteria / Robert A. Burne and Robert G. Quivey, Jr. -- 18. Identification of Bacterial Cell-Surface Virulence Determinants with TnphoA / Melissa R. Kaufman and Ronald K. Taylor -- 19. Temperature-Sensitive Mutants of Bacterial Pathogens: Isolation and Use to Determine Host Clearance and in Vivo Replication Rates / Anne Morris Hooke -- 20. Use of Conditionally Counterselectable Suicide Vectors for Allelic Exchange / Scott Stibitz -- 21. In Vivo Expression Technology for Selection of Bacterial Genes Specifically Induced in Host Tissues / James M. Slauch, Michael J. Mahan and John J. Mekalanos -- 22. Zymographic Techniques for Detection and Characterization of Microbial Proteases / Marilyn S. Lantz and Pawel Ciborowski -- 23. ADP-Ribosylating Toxins / Luciano Passador and Wallace Iglewski -- 24. Assays of Hemolytic Toxins / Gail E. Rowe and Rodney A. Welch -- 25. Assay of Cytopathogenic Toxins in Cultured Cells / Monica Thelestam and Inger Florin -- 26. Use of Lipid Bilayer Membranes to Detect Pore Formation by Toxins / Bruce L. Kagan and Yuri Sokolov -- 27. Uptake and Processing of Toxins by Mammalian Cells / Catharine B. Saelinger and Randal E. Morris -- 28. Induction of Cytokines in Mononuclear and Vascular Cells by Endotoxin and Other Bacterial Products / Harald Loppnow, Helmut Brade and Ernst Theodor Rietschel [et al.] -- 29. Effect of Bacterial Products on Colony-Stimulating Factor Production / Melinda S. Detrick, Robert Kreisberg and Robert N. Moore -- 30. Effect of Bacterial Products of Tumor Necrosis Factor Production: Quantitation in Biological Fluids or Tissues / Zell A. McGee and Christopher M. Clemens -- 31. Induction of Prostaglandin Release from Macrophages by Bacterial Endotoxin / Ken-ichi Tanamoto -- 32. Effect of Bacterial Products on Interferon Production / Gerald Sonnenfeld -- 33. Measurement of Nonopsonic Phagocytic Killing by Human and Mouse Phagocytes / Richard F. Rest and David P. Speert -- 34. Measurement of Opsonic Phagocytosis by Human Polymorphonuclear Neutrophils / John R. Kalmar -- 35. Measurement of Human Neutrophil Respiratory Burst Activity during Phagocytosis of Bacteria / Richard F. Rest -- 36. Complement-Meditated Bacterial Killing Assays / Daniel P. McQuillen, Sunita Gulati and Peter A. Rice -- 37. Measurement of Phagosome-Lysosome Fusion and Phagosomal pH / Thomas H. Steinberg and Joel A. Swanson -- 38. Neutrophil Defensins: Purification, Characterization, and Antimicrobial Testing / Sylvia S. L. Harwig, Tomas Ganz and Robert I. Lehrer -- 39. Bacterial Lectinlike Adhesins: Determination and Specificity / Janina Goldhar -- 40. Possible Interaction between Animal Lectins and Bacterial Carbohydrates / Robert E. Mandrell, Michael A. Apicella and Raghar Lindstedt [et al.] -- 41. Isolation and Identification of Eukaryotic Receptors Promoting Bacterial Internalization / Guy Tran Van Nhieu and Ralph R. Isberg -- 42. Interactions of Bacteria with Leukocyte Integrins / Eva Rozdzinski and Elaine Tuomanen -- 43. Solid-Phase Binding of Microorganisms to Glycolipids and Phospholipids / Carolyn D. Deal and Howard C. Krivan -- 44. Characterization of Microbial Host Receptors / Per Falk, Thomas Boren and Staffan Normark -- 45. Measurement of Invasion by Gentamicin Resistance / Eric A. Elsinghorst -- 46. Measurements of Invasion by Antibody Labeling and Electron Microscopy / Jos P. M. Van Putten, Jan F. L. Weel and Heike U. C. Grassme -- 47. Polarized Epithelial Monolayers: Model Systems to Study Bacterial Interactions with Host Epithelial Cells / M. Graciela Pucciarelli and B. Brett Finlay -- 48. Interjunctional Invasion of Endothelial Cell Monolayers / David A. Haake and Michael A. Lovett -- 49. Intracellular Growth of Bacteria / Sian Jones and Daniel A. Portnoy -- 50. Inhibitors of Cytoskeletal Function and Signal Transduction to Study Bacterial Invasion / Ilan Rosenshine, Sharon Ruschkowski and B. Brett Finlay -- 51. Methods to Visualize Actin Polymerization Associated with Bacterial Invasion / Lewis G. Tilney and Mary S. Tilney -- 52. Measurement of Free Intracellular Calcium Levels in Epithelial Cells as Consequence of Bacterial Invasion / John L. Pace and Jorge E. Galan -- 53. Shigella flexneri: Isolation of Noninvasive Mutants of Gram-Negative Pathogens / Robert Menard and Philippe J. Sansonetti -- 54. Isolation of Salmonella Mutants Defective for Intracellular Survival / Frances Bowe and Fred Heffron -- 55. Small Plaque Mutants / Sian Jones and Daniel A. Portnoy.