1. An introduction to enzymes -- 2. The structure of proteins -- 3. The biosynthesis and properties of proteins -- 4. Specificity of enzyme action -- 5. Monomeric and oligomeric enzymes -- 6. An introduction to bioenergetics, catalysis and kinetics -- 7. Kinetics of single-substrate enzyme-catalysed reactions -- 8. Enzyme inhibition -- 9. Kinetics of multi-substrate enzyme-catalysed reactions -- 10. The investigation of active site structure -- 11. The chemical nature of enzyme catalysis -- 12. The binding of ligands to proteins -- 13. Sigmoidal kinetics and allosteric enzymes -- 14. The significance of sigmoidal behaviour -- 15. Investigation of enzymes in biological preparations -- 16. Extraction and purification of enzymes -- 17. Enzymes as analytical reagents -- 18. Instrumental techniques available for use in enzymatic analysis -- 19. Some applications of enzymatic analysis in medicine and industry -- 20. Biotechnological applications of enzymes.

Griffiths, David.